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KMID : 0613820130230010102
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Journal of Life Science
2013 Volume.23 No. 1 p.102 ~ p.109
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Isolation and Identification of GABA-producing Microorganism from Chungkookjang
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Mann So-Yon
Kim Eun-Ah
Lee Ga-Young
Kim Ro-Ui
Hwang Dae-Youn
Son Hong-Joo
Kim Dong-Seob
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Abstract
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To isolate GABA-producing microorganisms, 1,500 strains were isolated from different Chungkookjang samples and screened. From these strains, 20 were selected for further analyses based on a protease and slime-producing activity test. The MC 31 strain showed the highest GABA concentration in Chungkookjang and was used in this study. MC 31 was identified as Bacillus subtilis by an API 50CHB kit and 16S rDNA sequences analysis and named as B. subtilis MC 31. B. subtilis MC 31 showed exponential growth up to 12 hours at 37℃ in LB broth, and it reached a stationary phase after 24 to 36 hours of incubation. B. subtilis MC 31 showed maximum GABA content at 72 hours after incubation at 40℃.
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KEYWORD
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Isolation, identification, GABA, Chungkookjang
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